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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H, M, B, Chp | WB | Rb | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-886 |
| Replaces | 05-886 |
| Description | Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W, rabbit monoclonal |
| Alternate Names |
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| Background Information | The STAT proteins (signal transducer and activator of transcription) comprise a family of transcription factors. STATs are activated through tyrosine phosphorylation, mainly by JAK kinases, which results in their dimerization, nuclear translocation and regulation of gene expression. STAT5 activity is induced by numerous cytokines and growth factors. While STAT5A and STAT5B share 96% amino acid homology, the two isoforms have distinct physiological functions. Phosphorylation of tyrosine 694/699 is a key marker of STAT5A/B activation. |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Control |
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| Presentation | Cultured supernatant containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect phospho-STAT5A/B (Tyr694/699) using this Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W validated for use in WB. |
| Key Applications |
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| Applications Not Recommended |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide containing the sequence DGpYVK in which pY corresponds to phospho-tyrosine at residue 694 of human STAT5A or residue 699 of STAT5B. |
| Epitope | pTyr694/699 |
| Clone | A11W |
| Host | Rabbit |
| Specificity | Phospho-STAT5A/B (Tyr694/699). |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq] |
| Gene Symbol |
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| Modifications |
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| UniProt Number |
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| UniProt Summary | FUNCTION: Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. SUBUNIT: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 By similarity. Interacts with NCOA1 and SOCS7. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note= Translocated into the nucleus in response to phosphorylation. PTM: Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity By similarity. SIMILARITY: Belongs to the transcription factor STAT family. Contains 1 SH2 domain. |
| Molecular Weight | ~95 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by western blot on RIPA lysates from 3T3/NIH cells untreated and treated with PDGF. Western Blot Analysis: A 1:1,000-1:2,000 dilution of this antibody detected phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |