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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Ch, Av | IHC, IP, NEUT, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-172 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-IGF-I Antibody, clone Sm1.2 |
| Alternate Names |
|
| Background Information | Insulin-like growth factor 1 (IGF-1) is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 is produced primarily by the liver as an endocrine hormone and targets tissues in a paracrine/autocrine fashion. Production is stimulated by growth hormone and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5b. Approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. Its primary action is mediated by binding to specific IGF receptors present on many cell types in many tissues. The signal is transduced by intracellular events. IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse IgG1κ lyophilized powder, lyophilized from 105 μL of 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-IGF-I Antibody, clone Sm1.2 is an antibody against IGF-I for use in IH, IP, NEUT & WB. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: 5 μg of a previous lot immunoprecipitated 100 ng of IGF-I. Immunohistochemistry: 10 μg/mL of a previous lot was reported by an independent laboratory to detect IFG-I in formalin-fixed, paraffin-embedded skin sections. (Lui, 1994). Neutralization: 10-20 μg/mL of a previous lot inhibited the activity of 10 ng/mL of IGF-I as determined by testing with chicken embryo fibroblasts (CEF) using an ATP endpoint assay (ATPLiteTM-M, Packard Instruments). |
| Biological Information | |
|---|---|
| Immunogen | Human insulin-like growth factor I (IGF-I) purified from human plasma. (van Wyk, J.J., 1986). |
| Clone | Sm1.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | IGF-I. (Russel, W.E., 1984). 40% cross reactivity to IGF-II. (van Wyk, J.J., 1997). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, mouse, rat and possibly chicken. Occasional difficulty neutralizing rat IGF-I due to binding protein interference. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P05019 # The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity.| P01343 # The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. SIZE: 195 amino acids; 21841 Da SUBCELLULAR LOCATION: Secreted. DOMAIN: SwissProt: P05019 DISEASE: SwissProt: P05019 # Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.| P01343 # Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. SIMILARITY: SwissProt: P05019 ## Belongs to the insulin family.| P01343 ## Belongs to the insulin family. |
| Molecular Weight | 17 kDa, migrates to ~7 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated on IGF-I (Catalog # 01-208) under non-reducing conditions. Western Blot Analysis: 0.5-2 μg/mL of this antibody detected 100 ng of IGF-I (Catalog # 01-208) under non-reducing conditions. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |