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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ca, H, R | ICC, IHC, IH(P), WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5804 |
| Replaces | AB1540 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody |
| Alternate Names |
|
| Background Information | Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit serum. Liquid. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody is an antibody against Glial Fibrillary Acidic Protein (GFAP) for use in IC, IH, IH(P) with more than 65 product citations. |
| Key Applications |
|
| Application Notes | Immunohistochemistry(paraffin): Representative images from a previous lot. Immunocytochemistry: A 1:1,000 dilution of a previous lot was used in Immunocytochemistry. |
| Biological Information | |
|---|---|
| Immunogen | Purified bovine GFAP |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes Glial Fibrillary Acidic Protein (GFAP). |
| Species Reactivity |
|
| Species Reactivity Note | Human, rat, bovine and canine. It is expected that the antibody will react with most mammalian GFAP due to homology. Reactivity with other species has not been confirmed. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q28115 # GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. SIZE: 428 amino acids; 49512 Da SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments. SIMILARITY: SwissProt: Q28115 ## Belongs to the intermediate filament family. |
| Molecular Weight | 50 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunohistochemistry on brain tissue, astrocytes, and neurons. Immunohistochemistry(paraffin) Analysis: GFAP (IHC2078) staining of Human Brain. Tissue pretreated with Citrate Buffer, pH 6.0. Pre-diluted polyclonal antibody, using IHC-Select® Detection with HRP-DAB. Glial cells stain strongly (brown). Optimal Staining with Citrate Buffer Epitope Retrieval: Human Brain |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |