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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IF | M | FITC | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1759F |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-CD59 Antibody, clone 2/24, FITC conjugated |
| Overview | BIOCHEMISTRY: p I = 6.1 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | FITC |
| Presentation | Purified immunoglobulin. FITC Conjugated. The antibody is supplied in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD59 Antibody, clone 2/24, FITC conjugated detects level of CD59 & has been published & validated for use in FC & IF. |
| Key Applications |
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| Application Notes | This monoclonal is useful in studies of complement and homologous restriction of complement action of the properties of GPI linked proteins and in the characterisation of cells from patients with PNH. SUGGESTED USAGE: Flow cytometry and immunofluorescence – use 10µL direct from the vial per 1x106 cells in a volume of 100 µL. For analysis of erythrocytes, whole blood samples should be diluted approximately 500 fold. For nucleated cells, use of the buffer coat will remove the burden of erythrocytes. Platelets may be harvested by low speed centrifugation. |
| Biological Information | |
|---|---|
| Immunogen | Human erythrocytes |
| Clone | 2/24 |
| Host | Mouse |
| Specificity | This antibody binds to a 17-25kD glycoprotein (Fletcher et al., 1990a,b) which is distributed on human cells and has a glycosyl-phosphatidylinositol (GPI) linkage to the plasma membrane(Hadam, 1989). It is identical to other CD59 monoclonals, MEM-43 and YTH 53.1, defined at the 4th International Conference on Human Leucocyte Differentiation Antigens(Davies et al., 1989; Stefanova et al., 1989). CD59 is an inhibitor of attack by autologous complement(Ojcius et al., 1990) possibly by limiting incorporation of C9 into the membrane complex(Rollins & Sims, 1990; Meri et al., 1990). GPI linked proteins such as CD59 are deficient on cells from patients with the acquired clonal disorder paroxysmal nocturnal haemoglobulinuria (PNH) in which the erythrocytes are susceptible to lysis by complement and leading to anemia(Rosse, 1990). Evidence suggests that CD59 may be the most important complement regulatory protein missing in PNH(Fletcher et al., 1990; Yamashina et al., 1990). Cell reactivity (Fletcher et al., 1990a,b; Hadam, 1989): The antibody reacts with all leucocytes, platelets, fibroblasts, erythrocytes and most cell lines. It is negative on U937 and Daudi cell lines. It is reduced on affected cells from patients with PNH with a distribution reflecting the clonal nature of the disease. |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P13987 # Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. SIZE: 128 amino acids; 14177 Da SUBUNIT: Interacts with T-cell surface antigen CD2. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. PTM: N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. & Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. SIMILARITY: SwissProt: P13987 ## Contains 1 UPAR/Ly6 domain. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | STORAGE Store at 2 to 8oC, for up to 6 months. For prolonged periods, store below -20oC in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES. WARNING The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water. |
| Packaging Information | |
|---|---|
| Material Size | 100 assays |