We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Cell Signaling Dna Polymerase Eta (E1i7t) Rabbit mAb
List Price
$342.39
Your Price
$342.39
Cell Signaling Dna Polymerase Eta (E1i7t) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-13848S
MFG.PART:
13848S
UNSPSC:
41116111
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 80 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | DNA Polymerase η (E1I7T) Rabbit mAb recognizes endogenous levels of total POLH protein. The antibody recognizes a 40 kDa background band of unknown origin. In some cell lines, the antibody recognizes a 60 kDa band of unknown origin. This band does not correspond to the expected size of truncated POLH in GM03617 cells. |
| Species Reactivity | Human |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro309 of human POLH protein. |
| Background | A total of fifteen mammalian DNA polymerase enzymes catalyze the synthesis of nascent DNA during DNA replication and repair (1). DNA polymerase eta (POL η, POLH, Rad30) is one of a specialized type of DNA polymerases that function in DNA repair and translesion synthesis (TLS). POLH can accommodate and read through bulky DNA lesions such as pyrimidine dimers, which allows for continued DNA synthesis past lesions and limited stalling of replication forks (2,3). Damage inducing conditions, such as exposure to UV light or cisplatin, recruit POLH to sites of bulky DNA lesions where the polymerase interacts with PCNA (4,5). Mutations in the human POLH gene can result in a form of xeroderma pigmentosum (XPV), an autosomal recessive disorder characterized by hypersensitivity to light and susceptibility to skin cancer (6). |
| SKU | CSIG-13848S |
|---|---|
| Featured | No |
| Supplier Part Number | 13848S |
| UM | EA |
| UNSPSC | 41116111 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |