Cell Signaling Phospho-Aurora A (Thr288) (C39d8) Rabbit mAb

Name: Cell Signaling Phospho-Aurora A (Thr288) (C39d8) Rabbit mAb
SKU: CSIG-3079T
Price: 164.63 USD

List Price $164.63 Your Price $164.63

Cell Signaling Phospho-Aurora A (Thr288) (C39d8) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3079T

MFG.PART: 3079T

UNSPSC: 41116111

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	48
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:1600}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb detects endogenous levels of Aurora A only when phosphorylated at Thr288.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr288 of human Aurora A.
Background	Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).

Specifications