Sigma-Aldrich Bovine Serum Albumin Fraction V, Fatty Acid Free,Fr

Biological Source

bovine

Quality Level

100

Assay

98.5% (electrophoresis)

Form

lyophilized

Mol Wt

M_r68 kDa

Packaging

pkg of 50 g

Manufacturer/Tradename

Roche

Origin

USA origin

Technique(S)

immunofluorescence: suitable
ligand binding assay: suitable

Impurities

Immunoglobulins, none detected
Triglycerides, none detected
≤0.2 mg/g fatty acids

Related Categories

Albumins and Transport Proteins

Bovine Serum Albumin

Roche® Life Science Products

General Description

Highest quality for fatty acid and lipid research.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.

Application

Bovine Serum Albumin, Fraction V, fatty acid free is used:

• in lipid research to study lipid metabolism in biological systems
• to identify transport mechanisms in living organisms
• to study ligand binding
• to investigate ionic binding
• in the preparation of solutions during isolation of cardiomyocytes
• as a blocking solution to incubate embryos for immunofluorescence
• in dual polarisation interferometry (DPI) to study the interaction between membrane active peptides and artificial bilayers[5]

Biochem/Physiol Actions

The fatty acids that are insoluble in circulating plasma are transported primarily by serum albumin. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.[6]

Features And Benefits

• Free from fatty acids according to current quality control procedures (suitable for lipid research)
• High quality
• Low content of heavy metal ions
• Long-term stability

Quality

Purity:≥98.5 % albumin (electrophoresis), 98% protein
Contaminants:<0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), ≤5% H2O,≤30 ppm heavy metals

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

Wgk_Germany

WGK 1

Flash_Point_F

Not applicable

Flash_Point_C

Not applicable