Sigma-Aldrich Endoproteinase Glu-C Seque

Biological Source

Staphylococcus aureus(V8)

Form

lyophilized (salt-free)
lyophilized

Specific Activity

≥15 units/mg protein

Mol Wt

M_r27 kDa

Packaging

pkg of 3 × 50 μg (11047817001)
pkg of 50 μg (11420399001)

Manufacturer/Tradename

Roche

Optimum pH

4.0-7.8

Storage Temp.

2-8°C

Related Categories

Proteases

Roche® Life Science Products

General Description

Endoproteinase Glu-C Sequencing Grade is a serine protease isolated fromStaphylococcus aureusV8 as a highly purified and specific protease. Inhibitors of Endoproteinase Glu-C are DFP, α2-macroglobulin and TLCK.

Specificity

Serine protease that very specifically hydrolyzes peptide bonds at the carboxylic side of Glu (in ammonium bicarbonate, pH 7.8, or ammonium acetate buffer, pH 4.0), or Glu and Asp (in phosphate buffer, pH 7.8). The specificity of Endoproteinase Glu-C Sequencing Grade is verified with the oxidized B-chain of insulin (insulin B_ox) as substrate. High concentrations of Endoproteinase Glu-C Sequencing Grade (1 part by weight enzyme with 10 parts by weight insulin B_ox) are incubated to detect traces of impurities, for example, contaminating proteases.
Heat inactivation:Most proteases sequencing grade are stopped by boiling for 5 minutes.

Application

Use Endoproteinase Glu-C Sequencing Grade for protein structure analysis and for sequence analysis.

Features And Benefits

Free of impurities that may interfere with separation of peptides in RP HPLC.

Preparation Note

Working concentration:1/100 to 1/20 (w/w)
With a minimum amount of 1 to 5 μg insulin B_ox,sufficient levels can be detected using the Column Aquapore RUP 300, 4.6 x 100 nm, 7 μm.
Working solution:Solvent is recommended in double-distilled water.
Solvents other than water have not been tested; Roche recommends using 50 mm HEPES, pH 7.8.
Storage conditions (working solution):+2°C to +8°C for 1-2 days.
Stability:At +2 to +8°C within specification range for 12 months; stored dry.

Reconstitution

Lyophilized Endoproteinase Glu-C sequencing grade is reconstituted in double-dist. water. To avoid autolysis, the incubation temperature should not exceed 25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

GHS07,GHS08

Signalword

Danger

Hcodes

H315 - H319 - H334 - H335

Pcodes

P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target_Organs

Respiratory system

Storage Class

11 - Combustible Solids

Wgk_Germany

WGK 1

Flash_Point_F

does not flash

Flash_Point_C

does not flash