Sigma-Aldrich Anti-Digoxigenin-Pod (Poly

Biological Source

sheep

Quality Level

100

Conjugate

peroxidase conjugate

Antibody Form

purified immunoglobulin

Antibody Product Type

primary antibodies

Clone

polyclonal

Form

lyophilized

Packaging

pkg of 50 U

Manufacturer/Tradename

Roche

Isotype

IgG

Related Categories

Nucleic Acid Labeling

Roche® Life Science Products

General Description

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems.Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to polymerized horseradish peroxidase.

Specificity

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Application

Anti-Digoxigenin-POD (poly), Fab fragments has been used for the detection of digoxigenin-labeled compounds using:

• whole-mount in situ hybridization
• fluorescence in situ hybridization
• enzyme linked immunosorbent assay (ELISA)[5]
• DOT blot
• Southern blot
• western blot
• double labeling experiments
• lectin blots

Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.
Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.

Preparation Note

Working concentration:Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:

• DOT blot: 50 to 100 mU/mL
• ELISA: 2 to 50 mU/mL
• Western blot: 50 to 100 mU/mL
• Southern blot: 50 to 100 mU/mL

Working solution:100 mm Tris-HCl, 150 mm NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Reconstitution

Add 1 mL double-distilled water to a final concentration of 50 U/mL.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

GHS07

Signalword

Warning

Hcodes

H317

Pcodes

P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

Wgk_Germany

WGK 2

Flash_Point_F

does not flash

Flash_Point_C

does not flash