Sigma-Aldrich G418 Solution 100 mL

Quality Level

100

Assay

98% (TLC)

Form

solution

Mol Wt

692.7 Da

Packaging

pkg of 100 mL (04727894001 [5 x 20 mL, equivalent to 5 g])
pkg of 20 mL (04727878001 [equivalent to 1 g])

Manufacturer/Tradename

Roche

Concentration

50 mg/mL

Technique(S)

transfection: suitable

Antibiotic Activity Spectrum

protozoa

Mode Of Action

protein synthesis | inhibits

Related Categories

Antibiotics

Roche® Life Science Products

Transfection

General Description

O-2-Amino-2,7-dideoxy-δ-glycero-α-δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-δ-streptamine, disulfate salt g-418, an aminoglycoside antibiotic, is used as a dominant selection agent in transfection experiments. The antibiotic interferes with the function of 80S ribosomes and blocks protein synthesis in eukaryotic cells. Transfection of neomycin-resistance genes into cells results in resistance to g-418 and enables the cells to grow in culture media containing g-418.

Application

g-418 Solution is used to select eukaryotic cells that are stably transfected with the neomycin resistance gene (neo), and to maintain the phenotype (neo^r) of resistant cells.
g-418 is also used to eliminate contaminating fibroblasts from mixed cultures.

Biochem/Physiol Actions

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.

Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.

Features And Benefits

• Convenient sterile-filtered solution for immediate use – saves valuable time and effort, and avoids potential errors arising from the preparation of sterile working stock solution from g-418 powder.
• Consistent quality and potency – shows no lot-to-lot variation in its ability to select resistant cells.
• Function tested in a cell culture assay - ensures effectiveness.
• Very pure, as indicated by TLC and microbiological assays – allows you to use less antibiotic while producing greater selection pressure. Lack of contaminants means minimal toxicity.
• Cost effective, since the selected colonies are stable.

Contents
Aqueous solution of g-418, 892 μg/mg, filtered through 0.2μm pore size membrane

Quality

Purity:= 98%, as shown by TLC.
Function testing:in cell culture.

Preparation Note

Working concentration:The optimal concentration of g-418 must be determined experimentally and varies with the cell type used.
Active weight:>700μg/mg
Recommended concentration in cell culture:The optimal concentration of g-418 must be determined experimentally; it varies from 100μg/mL to 1mg/mL, depending on the cell type used.
Specific rotation:104° to 121°, in water (on dry basis, according to USP XXII).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

GHS08

Signalword

Danger

Hcodes

H317 - H334

Pcodes

P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

Wgk_Germany

WGK 1

Flash_Point_F

No data available

Flash_Point_C

No data available