Sigma-Aldrich Hif Inhibitor V 1pc X 10mg

Assay

≥99% (HPLC)

Quality Level

100

Form

powder

Manufacturer/Tradename

Calbiochem^®

Storage Condition

OK to freeze
protect from light

Color

white

Solubility

DMSO: 50 mg/mL

Storage Temp.

2-8°C

General Description

A cell-permeable phenoxyacrylamido compound that inhibits hypoxia-induced cellular HIF-1α accumulation in a manner similar to LW6 (Cat. No. 400083) by upregulating von Hippel-Lindau tumor suppressor gene product pVHL, but with much higher potency (IC₅₀= 120 nM vs 2.44 µM, respectively, against hypoxia-induced HRE reporter activity in HCT116 cells). Shown to suppress the mRNA levels of HIF-1 target genes in HCT116 cultures (VEGF,EPO, andMMP-9; effective conc. ≥2 µM) and effectively inhibit HUVEC tubule formation (effective conc. 2 to 5 µM) under hypoxia conditions.A cell-permeable phenoxyacrylamido compound that inhibits hypoxia-induced cellular HIF-1α accumulation in a manner similar to LW6 (Cat. No. 400083) by upregulating Hippel-Lindau tumor suppressor gene product pVHL, but with much higher potency (IC₅₀= 120 nM vs 2.44 µM, respectively, against hypoxia-induced HRE reporter activity in HCT116 cells). Shown to suppress HIF-1 target genes expression in HCT116 cultures and inhibit HUVEC tubule formation (effective conc. ≥2 µM) under hypoxia conditions.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Biochem/Physiol Actions

Cell permeable: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.Use only fresh DMSO for reconstitution.

Other Notes

Naik, R., et al. 2012.J. Med. Chem. 55,10564.CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany