Sigma-Aldrich Insolution Phorbol-12-Myri

Quality Level

100

Assay

≥98% (HPLC)

Form

liquid

Manufacturer/Tradename

Calbiochem^®

Storage Condition

OK to freeze
avoid repeated freeze/thaw cycles
desiccated (hygroscopic)
protect from light

Storage Temp.

−70°C

Inchi

1S/C36H56O8/c1-7-8-9-10-11-12-13-14-15-16-17-18-29(39)43-32-24(3)35(42)27(30-33(5,6)36(30,32)44-25(4)38)20-26(22-37)21-34(41)28(35)19-23(2)31(34)40/h19-20,24,27-28,30,32,37,41-42H,7-18,21-22H2,1-6H3/t24-,27+,28-,30-,32-,34-,35-,36-/m1/s1

Inchi Key

PHEDXBVPIONUQT-RGYGYFBISA-N

General Description

Most commonly-used phorbol ester. Extremely potent mouse skin tumor promoter. Activates protein kinase Cin vivoandin vitro, even at nM concentrations. Promotes the expression of iNOS in cultured hepatocytes. Activates Ca²⁺-ATPase and potentiates forskolin (>Cat. No. 344270, >Cat. No. 344282)-induced cAMP formation. Inhibits apoptosis induced by the Fas antigen, but induces apoptosis in HL-60 promyelocytic leukemia cells. Its binding is reversible.

Biochem/Physiol Actions

Cell permeable: yesPrimary Target
PKCReversible: yesSecondary Target
Ca²⁺-ATPase

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Physical Form

A 10 mm (2 mg/324 µL) solution of Phorbol-12-myristate-13-acetate (Cat. No. 524400) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Use only fresh DMSO for preparing further dilutions.

Other Notes

Powell, D.J., et Al. 2003.Mol. Cell Biol. 23,7794.
Tepper, C.g., et Al. 1995.Proc. Natl. Acad. Sci. USA 92,8443.
Oishi, K., and Yamaguchi, M. 1994.J. Cell. Biochem. 55,168.
Macfarlane, D.E., and O′Donnell, P.S. 1993.Leukemia 7,1846.
Ghandi, V.C. and Jones, D.J., 1992.Neuropharmacol. 31,1101.
Hortelano, S., et Al. 1992.J. Biol. Chem. 267,24937.
Kontny, E., et Al. 1992.Eur. J. Pharmacol. 227,333.
Zanaboni, P.B., et Al. 1992.J. Appl. Physiol. 73,2011.
Saltis, J., et Al. 1991.J. Biol. Chem. 266,261.
Beguinot, L., et Al. 1985.Proc. Natl. Acad. Sci. USA 82,2774.
Perchellet, J. 1985.Carcinogenesis 6,567.
Nishizuka, Y. 1984.Science 255,1365.
Mastro, A. 1982.Lymphokines 6,263.CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyGHS06,GHS08H311 + H331 - H315 - H317 - H319 - H334 - H351P202 - P280 - P302 + P352 + P312 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313