Sigma-Aldrich Autophagy Inhibitor IV Cpd

Assay

≥98% (HPLC)

Quality Level

100

Form

solid

Potency

0.67 mm IC₅₀

Manufacturer/Tradename

Calbiochem^®

Storage Condition

OK to freeze
protect from light

Color

white

Solubility

DMSO: 50 mg/mL

Storage Temp.

2-8°C

General Description

A cell-permeable 3-methyladenine (3-MA) derivative that acts as an autophagy inhibitor via the inhibition of class III PI3K, and without touching class I PI3K. Shown to block 80% of starvation - induced autophagy at 1 mm (IC₅₀= 0.67 mm, compared with 3-MA at 6 mm). Displays much better solubility than 3-MA (5 times more soluble in water and 30 times more soluble in DMSO). Also found to reduce cell viability by 55% at 1 mm at 12 hours compared with 72% reduction for 3-MA at 10 mm.A cell-permeable 3-methyladenine (3-MA) derivative that acts as an autophagy inhibitor via the inhibition of class III PI3K, and without touching class I PI3K. Shown to block 80% of starvation-induced autophagy at 1 mm (IC₅₀= 0.67 mm, compared with 3-MA at 6 mm). Displays much better solubility than 3-MA (5 times more soluble in water and 30 times more soluble in DMSO). Also found to reduce cell viability by 55% at 1 mm at 12 hours compared with 72% reduction for 3-MA at 10 mm.

Please note that the molecular weight for this compound is lot-specific due to variable water content.

Biochem/Physiol Actions

Primary Target
PI3KReversible: yes

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Wu, Y., et Al, 2013.Autophagy 9,595.CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany