Sigma-Aldrich Insolution Calyculin A Dis

Quality Level

100

Assay

≥98% (HPLC)

Form

liquid

Manufacturer/Tradename

Calbiochem^®

Storage Condition

OK to freeze
avoid repeated freeze/thaw cycles
desiccated (hygroscopic)
protect from light

Storage Temp.

−70°C

Inchi

1S/C50H81N4O15P/c1-29(20-22-51)16-14-17-30(2)32(4)24-33(5)42(57)35(7)38(55)25-41(65-13)45-46(69-70(61,62)63)49(8,9)50(68-45)26-39(56)34(6)40(67-50)19-15-18-36-27-66-48(53-36)31(3)21-23-52-47(60)44(59)43(58)37(28-64-12)54(10)11/h14-18,20,24,27,31,33-35,37-46,55-59H,19,21,23,25-26,28H2,1-13H3,(H,52,60)(H2,61,62,63)/b16-14+,18-15+,29-20-,30-17+,32-24+/t31-,33+,34+,35+,37-,38-,39-,40+,41-,42+,43-,44-,45+,46-,50-/m0/s1

Inchi Key

FKAWLXNLHHIHLA-QJLNTFFJSA-N

General Description

Cell-permeable protein phosphatase 2A (PP2A) inhibitor (IC₅₀= 0.5-1.0 nM). Also inhibits protein phosphatase 1 (PP1; IC₅₀= 2 nM). A non-phorbol ester type tumor promoter that induces oxidative DNA damage. Selectively increases the efficacy of AMPA receptor-mediated synaptic transmission at excitatory synapses. Recently, it has been used to study the regulation of Ca²⁺-activated K⁺channels in smooth muscle and to correlate shape changes in fibroblasts with the degree of protein phosphorylation. Also known to prevent γ-radiation induced apoptosis in Burkitt′s lymphoma cell line BM13674.

Biochem/Physiol Actions

Cell permeable: yesPrimary Target
protein phosphatase-2A & -1Reversible: yesTarget IC₅₀: 0.5-1.0 and 2 nM for PP2A and PP1, respectively

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Physical Form

A 100 µM (5 µg/50 µL) sterile-filtered solution of Calyculin A,Discodermia calyx(Cat. No. 208851) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C. Use only fresh DMSO for further dilutions.

Other Notes

Murakami, N., et Al. 1994.Neurosci. Lett. 176,181.
Takeuchi, T., et Al. 1994.Biochem. Biophys. Res. Commun. 205,1803.
Figurov, A., et Al. 1993.Eur. J. Neurosci. 5,1083.
Song, Q., and Lavin, M.F. 1993.Biochem. Biophys. Res. Commun. 190,47.
Gopalakrishna, R., et Al. 1992.Biochem. Biophys. Res. Commun. 189,950.
Carl, A., et Al. 1991.Am. J. Physiol. 261,C387.
Suganuma, M., et Al. 1990.Cancer Res. 50,3521.
Cohen, P. 1989.Annu. Rev. Biochem. 58,453.
Cohen, P., et Al. 1989.J. Biol. Chem. 264,21435.
Ishihara, H., et Al. 1989.Biochem. Biophys. Res. Commun. 159,871.
Kato, Y., et Al. 1988.J. Org. Chem. 53,3930.
Kato, Y., et Al. 1986.J. Am. Chem. Soc. 108,2780.CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany