Sigma-Aldrich Hsp90a Inhibitor Pu-29f

Assay

≥98% (HPLC)

Quality Level

100

Form

powder

Manufacturer/Tradename

Calbiochem^®

Storage Condition

OK to freeze
protect from light

Color

off-white

Solubility

DMSO: 100 mg/mL

Storage Temp.

2-8°C

General Description

A cell-permeable 6-amino-purine based compound that selectively binds to HSP90a (EC₅₀= 5.4 µM) and inhibits its activity in SKBr3 and MCF-7 breast cancer cells. Exhibits much reduced activity towards other paralogs (EC₅₀= 22.85, 71, and 173 5.4 µM for HSP90b, GRP94 AND Trap-1, respectively). Selectively depletes cytosolic HER2 and induces its redistribution towards lysosomes and early endosomes (~20 5.4 µM) without affecting membrane associated HER2. Does not appear to be toxic towards nonmalignant cells.A cell-permeable 6-amino-purine based compound that selectively binds to HSP90a (EC₅₀= 5.4 µM) and inhibits its activity in SKBr3 and MCF-7 breast cancer cells. Exhibits much reduced activity towards other paralogs (EC₅₀= 22.85, 71, and 173 5.4 µM for HSP90b, GRP94 AND Trap-1, respectively). Selectively depletes cytosolic HER2 and induces its redistribution towards lysosomes and early endosomes (~20 5.4 µM) without affecting membrane associated HER2. Does not appear to be toxic towards nonmalignant cells.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Biochem/Physiol Actions

Cell permeable: yesPrimary Target
HSP90a

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Patel, P.D., et al. 2013.Nat. Chem. Biol. 9,677.CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany