Sigma-Aldrich Orai1/Crac Blocker Cpd5j-4

Assay

≥98% (HPLC)

Quality Level

100

Form

powder

Manufacturer/Tradename

Calbiochem^®

Storage Condition

OK to freeze
protect from light

Color

off-white

Solubility

DMSO: 100 mg/mL

Storage Temp.

−20°C

General Description

A non-toxic thiourea derivative that acts as a blocker of Orai1, the pore subunit of Ca2+-release-activated Ca2+ (CRAC) in T cells. Exhibits a strong blocking effect on store-operated Ca2+ entry in HeLa-O+S cells and in Th17 cells. Preferentially reduces Th17 differentiation and ameliorates Th17-mediated autoimmune disease. Blocks the infiltration of T cells into the CNS and reduces the number of IL-17 producing cells. Also shown to reduce the expression of retinoic acid receptor-related orphan receptors a (RORa) and RORgt. Reduces the symptoms and delays the onset of experimental autoimmune encephalomyelitis (EAE) in myelin oligodendrocyte glycoproteins 35-55 (MOG35-55) immunized mice (2 mg/kg, i.p.).A non-toxic thiourea derivative that acts as a blocker of Orai1, the pore subunit of Ca²⁺-release-activated Ca²⁺(CRAC) in T cells. Exhibits a strong blocking effect on store-operated Ca²⁺entry in HeLa-O+S cells and in Th17 cells. Preferentially reduces Th17 differentiation and ameliorates Th17-mediated autoimmune disease. Blocks the infiltration of T cells into the CNS and reduces the number of IL-17 producing cells. Also shown to reduce the expression of retinoic acid receptor-related orphan receptors a (RORa) and RORgt. Reduces the symptoms and delays the onset of experimental autoimmune encephalomyelitis (EAE) in myelin oligodendrocyte glycoproteins 35-55 (MOG35-55) immunized mice (2 mg/kg, i.p.).

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Biochem/Physiol Actions

Primary Target
Orail1

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Kim, K., et Al. 2014.J Immunol. 192,110.CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany