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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-1036 |
| Description | Anti-March5 Antibody |
| Alternate Names |
|
| Background Information | Membrane-associated RING-CH protein 5 (March5), also known as NKP66, is thought to be a mitochondrial ubiquitin ligase that is localized on the mitochondrial membrane. March5 has a Plant Homeo-Domain (PHD) motif that is essential for E3 ubiquitin ligase action. MARCH 5 is also thought to have four transmembrane domains. March5 has been associated with mitochondrial fission and division. March5 also seems to have a defensive role against mitochondrial dysfunction by interacting with mutant superoxide dismutase 1 (mSOD1) and preventing mSOD1 from causing damage. mSOD1 damage has been linked to the onset and progression of amyotrophic lateral sclerosis. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit polyclonal serum containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-March5 Antibody is a Rabbit Polyclonal Antibody for detection of March5 also known as Membrane-associated RING finger protein 5 & has been validated in WB, ICC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot of this antibody was used by an independent laboratory in IC. (Korfali, N., et al. (2010). Mol Cell Proteomics. 9:2571-2585.) |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human March5. . |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human. Predicted to react with mouse and rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Summary: March5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM]. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1. PATHWAY: Protein modification; protein ubiquitination. SUBUNIT STRUCTURE: Monomer and homodimer. Interacts with MFN1, MFN2, DNM1L and FIS1. SUBCELLULAR LOCATION: Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane. Note: Authors show that the protein can be detected in endoplasmic reticulum. Authors show its presence only in mitochondria. TISSUE SPECIFICITY: Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle. DOMAIN: The RING-CH-type zinc finger domain is required for E3 ligase activity (by similarity). POST-TRANSLATIONAL MODIFICATION: Autoubiquitinated leading to degradation (short half-life). MISCELLANEOUS: By binding to and ubiquitinating two ALS1 variants of SOD1 (mSOD1 variants Arg-86 and Ala-94) it attenuates their cytotoxicity. SEQUENCE SIMILARITIES: Contains 1 RING-CH-type zinc finger. |
| Molecular Weight | ~52 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HL-60 cell lysate. Western Blot Analysis: A 1:2,000 dilution of this antibody detected March5 on 10 µg of HL-60 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |