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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1820 |
| Description | Anti-EEA1 Antibody |
| Alternate Names |
|
| Background Information | Early endosome antigen 1 (EEA1) is a 170 kDa coiled-coil protein belonging to a group of early endosomal Rab5 effector proteins. EEA1 has a putative role in the tethering/docking of endocytic vesicles prior to their fusion with early endosomes. This protein is characterized as containing a zinc finger-like domain and coiled-coil regions. The zinc finger-like domain, also known as the FYVE finger, is a motif rich in cystine, is a necessary component of EEA1’s ability for endosomal targeting, and is specifically known for its dual interaction with Rab5 GTPase and phosphotidylinositol 3-phosphate (Ptdlns-3-P). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1. |
| Key Applications |
|
| Application Notes | Western Blot (SNAP ID) Analysis: 1:250 dilution from a previous lot detected EEA1 on 10 µg of C2C12 cell lysate. Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected EEA1 in HeLa, A431, and C2C12 cells. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to the C-terminus of human EEA1. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes EEA1. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human and Mouse. Equine and Canine (99% sequence homology). Rat (98% sequence homology). Bovine (97% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. SUBUNIT STRUCTURE: Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. SUBCELLULAR LOCATION: Cytoplasm. Early endosome membrane; Peripheral membrane protein. DOMAIN: The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate. MISCELLANEOUS: Antibodies against EEA1 are found in sera from patients with subacute cutaneous lupus erythematosus and other autoimmune diseases. SEQUENCE SIMILARITIES: Contains 1 C2H2-type zinc finger. Contains 1 FYVE-type zinc finger. |
| Molecular Weight | ~ 170 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in BALB 3T3/A31 cell lysate. Western Blot Analysis: A 1:1000 dilution of this antibody detected EEA1 on 10 µg of BALB 3T3/A31 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |