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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 16-319 |
| Description | Anti-Rac1 Antibody, clone 23A8, Magnetic bead Conjugate |
| Alternate Names |
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| Background Information | Rac1 is a member of a RhoGTPase subfamily (Rac1-Rac4) that transduces extracellular signals from g-coupled protein receptors (GPCR), integrins and growth factor receptors to effector molecules that modulate multiple signaling pathways. While Rac1 supports the activation of MAPkinase pathways and gene activation, it’s most important functions include the regulation of cytoskeletal structures. Together with Rho (regulator of stress fibers) and Cdc42 (regulator of filopodia), Rac modulates the formation of focal adhesion (FA) complexes; membrane ruffles and lamellipodia that contribute to important cell functions related to cell attachment and movement. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | 20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 immunoprecipitation assays. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Rac1 Antibody, clone 23A8, Magnetic bead Conjugate detects level of Rac1 & has been published & validated for use in WB & IP. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | Recombinant protein containing the full length human Rac1. |
| Clone | clone 23A8 |
| Host | Mouse |
| Specificity | Recognizes human Rac1. |
| Isotype | IgG2b |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Other species have not been tested. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | Function: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. Enzyme regulation: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. Subunit structure: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, BAIAP2L1, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2. Interacts with NOXA1. Interacts with ARHGEF2. Interacts with NISCH By similarity. Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic sideBy similarity. Melanosome. Note= Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine By similarity. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue specificity: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. Domain: The effector region mediates interaction with DEF6. Sequence similarities: Belongs to the small GTPase superfamily. Rho family. |
| Molecular Weight | 21 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. DO NOT FREEZE! Wash the magnetic beads with an appropriate buffer prior to use to remove sodium azide. |
| Packaging Information | |
|---|---|
| Material Size | 400 µL |