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Radial glia play key roles in neuronal migration, axon guidance, and neurogenesis during development of the central nervous system. However, the molecular mechanisms regulating growth and morphology of these extended cells are unknown. ABBA, a novel member of the IRSp53/MIM protein family, is enriched in different types of radial glia. ABBA binds ATP-actin monomers with high affinity and deforms PIP2-rich membranes in vitro through its WH2 and IMdomains, respectively. In radial glia-like C6-R cells, ABBA localizes to the interface between the actin cytoskeleton and plasma membrane, and its depletion by RNAi leads to defects in lamellipodial dynamics and process extension. Together, ABBA acts as a novel regulator of actin and plasma membrane dynamics in radial glial cells, and provides evidence that the membrane binding/deformation activity is critical for the cellular functions of IRSp53/MIM/ABBA family proteins
Product Information
Format
Serum
Control
Mouse brain lysate
Presentation
Serum with with 0.05% NaN3
Quality Level
MQ100
Applications
Application
Anti-ABBA Antibody is an antibody against ABBA for use in WB, IC, IH.
Key Applications
Western Blotting
Immunocytochemistry
Immunohistochemistry
Application Notes
Non-Lot Specific Tested Application 1: Immunocytochemistry: 1:50 dilution in mouse primary glial cell line and C6, C6-R rat cell line.
Non-Lot Specific Tested Application 2: Immunohistochemistry: 1:1,000 in embryonic and adult brain tissue.
(See Reference for more information)
Biological Information
Immunogen
Recombinant protein.
Host
Rabbit
Specificity
Cat. # AB2209 recognizes ABBA
Species Reactivity
Human
Mouse
Rat
Species Reactivity Note
Human, Mouse and Rat. May react with Pan troglodytes and Macaca mulatta based on sequence homology. Reactivity with other species was not tested.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_941027
Gene Symbol
MGC79213
ABBA
UniProt Number
Q6P9S0
Molecular Weight
~82 kDa
Product Usage Statements
Quality Assurance
Routinely tested on mouse brain lysate Lot Specific Tested Application 1: Western Blot
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt.
