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Millipore Anti-Aquaporin 1 Antibody Detects Level Of Aquaporin 1 & Has Been Published & Validated For Use In Elisa, Wb, Ih. - Mill (Additional S&H or Hazmat Fees May Apply)
Water channel protein for red blood cells and kidney proximal tubule
Background Information
Aquaporin 1 is an integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Aquaporin 1 Antibody detects level of Aquaporin 1 & has been published & validated for use in ELISA, WB, IH.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Western Blotting: 1-10 μg/mL using Chemiluminescence technique. Immunoblots reveal two bands, a lower 28 kDa band corresponding to unglycosylated form and an upper high molecular 35-60 kDa glycosylated component (Denker et al. 1988; Smith & Agre 1991) We recommend the use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands. Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Aquaporin 1 in human kidney and mouse kidney tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the topological domain of rat Aquaporin 1.
Epitope
Topological domain
Concentration
1.0 mg/mL
Host
Rabbit
Species Reactivity
Human
Rat
Mouse
Species Reactivity Note
Human, Rat, Mouse. Predicted to react with Primate, Bovine, Sheep based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_036910
Gene Symbol
AQP1
Aquaporin-CHIP
MGC26324
AQP-CHIP
CO
Aquaporin-1
CHIP28
AQP-1
Purification Method
ImmunoAffinity Purified
UniProt Number
P29975
Molecular Weight
28 kDa
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human kidney tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Aquaporin 1 in 10 µg of human kidney tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
