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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2231 |
| Description | Anti-Dopamine Transporter; 100 µg Antibody |
| Alternate Names |
|
| Background Information | Plasmalemmal neurotransmitter transporters sequester synaptic and peri synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT), a 12 transmembrane domain transporter with the N and C terminal regions located within the cytoplasm is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration. The activity of the DAT reuptake carrier is sodium dependent, and it is suspected to play a role in such neurologic and psychiatric disorders as Parkinsons disease, Tourettes disease, schizophrenia, and addiction. DAT is also the target of several “DAT-blockers” including amphetamines and cocaine. These chemicals inhibit the action of DAT and, to a lesser extent, the other monoamine transporters, but their effects are mediated by separate mechanisms. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified Antibody in PBS with 0.1% NaN3 |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Dopamine Transporter, 100 µg Antibody is validated for use in WB for the detection of Dopamine Transporter, 100 µg. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Synthetic Linear Peptide |
| Epitope | N-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Cat. AB2231 recognizes the N-terminus of the reduced and non reduced forms of DAT. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The dopamine transporter (DAT1) mediates the active reuptake of dopamine from the synapse and is a principal regulator of dopaminergic neurotransmission. The DAT1 gene has been implicated in human disorders such as parkinsonism, Tourette syndrome, and substance abuse (Vandenbergh et al., 1992 [PubMed 1359373]).[supplied by OMIM] |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. SUBUNIT: Homooligomer; disulfide-linked. Interacts with PRKCABP and TGFB1I1. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. MISCELLANEOUS: This protein is the target of psychomotor stimulants such as amphetamines or cocaine. SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) family. |
| Molecular Weight | ~ 50 – 80 kDa Reduced form at approx 50 kDa and Non-reduced form at approx. 80 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinelyevaluated by western blot on rat brain lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Prior to removing the cap, gently centrifuge the vial and gently mix the solution. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |