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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | WB, IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2257 |
| Description | Anti-Vesicular GABA Transporter Antibody |
| Alternate Names |
|
| Background Information | Vesicular GABA transporter (VGAT), the first vesicular amino acid transporter to be molecularly identified, differs in structure from previously cloned vesicular neurotransmitter transporters and defines a novel gene family. VGAT is highly concentrated in the nerve endings of GABAergic neurons in the brain and spinal cord but also in glycinergic nerve endings. In contrast, hippocampal mossy fiber boutons, which are glutamatergic but known to contain GABA, lack VGAT immunoreactivity. Although the great majority of nerve terminals containing GABA or glycine are immunopositive for VGAT, subpopulations of nerve endings rich in GABA or glycine appear to lack the protein. Additional vesicular transporters or alternative modes of release may therefore contribute to the inhibitory neurotransmission mediated by these two amino acids. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit polyclonal serum in buffer containing 0.1M Tris-Glycine (pH7.4) 15mM NaCl with 0.05% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Vesicular GABA Transporter Antibody is an antibody against Vesicular GABA Transporter for use in WB, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: 1:50 - 1:500 dilution from a previous lot detected vesiluar GABA transporter using IHC-Select Detection with HRP-DAB. |
| Biological Information | |
|---|---|
| Immunogen | KLH conjugated Vesicular GABA Transporter corresponding to the C-terminus. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Cat. # AB2257 recognizes VGAT. |
| Species Reactivity |
|
| Species Reactivity Note | Rat. Predicted to react with human, rat and monkey. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq] |
| Gene Symbol |
|
| Purification Method | Antigen Affinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Involved in the uptake of GABA and glycine into the synaptic vesicles. SUBCELLULAR LOCATION:Cytoplasmic vesicle membrane; Multi-pass membrane proteinBy similarity. TISSUE SPECIFICITY: Retina. Expressed throughout the horizontal cells or more specifically at the terminals. Ref.5 SEQUENCE SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family. |
| Molecular Weight | Approx. 57 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on rat brain lysates. Western Blot Analysis: 1:500 - 1:1,000 dilution of this lot detected vesicular GABA transporter on 10 µg of rat brain lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |