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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2281 |
| Description | Anti-Na+K+Cl- Cotransporter 2 Antibody |
| Alternate Names |
|
| Background Information | The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henles loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. SLC12A1 is kidney specific and is essential in the regulation of ionic balance and cell volume. It is a component of an electrically silent transporter system, mediating sodium and chloride reabsorption. Defects in SLC12A1 are the cause of Bartter syndrome type 1, an autosomal recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Na+K+Cl-Cotransporter 2 Antibody detects level of Na+K+Cl-Cotransporter 2 & has been published & validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH conjugate followed by NA+K+CL- Cotransporter 2 corresponding to the cytoplasmic domain. |
| Epitope | Cytoplasmic domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the cytoplasmic domain of NA+K+CL- Cotransporter 2. |
| Species Reactivity |
|
| Species Reactivity Note | Proven to react with human, rat and mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henles loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Antigen Affinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY:Kidney specific. DISEASE: Defects in SLC12A1 are the cause of Bartter syndrome type 1 (BS1) [MIM:601678]. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS1 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS1 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia. Ref.1 SIMILARITIY: Belongs to the SLC12A transporter family. |
| Molecular Weight | ~150 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Western Blot Analysis: 1:2,000 dilution of this lot detected NA+K+CL- Cotransporter 2 on 10 µg of rat liver lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |