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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | IH(P), WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9983 |
| Description | Anti-Tyrosine Hydroxylase Antibody |
| Alternate Names |
|
| Background Information | Tyrosine Hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3 |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Tyrosine Hydroxylase Antibody detects level of Tyrosine Hydroxylase & has been published & validated for use in IH(P) & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:100 working dilutions were used on rat and mouse brain (paraffin embedded tissue). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Cat. # AB9983 recognizes the C-terminus of Tyrosine Hydroxylase |
| Species Reactivity |
|
| Species Reactivity Note | Mouse and Rat. Reactivity with other species has not been determined. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq] |
| Gene Symbol |
|
| Purification Method | Antigen Affinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe2+ ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands. INVOLVEMENT IN DISEASE: Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism. SEQUENCE SIMILARITIES: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. |
| Molecular Weight | ~ 58 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |