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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | WB, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE149 |
| Description | Anti-ASF1a Antibody |
| Alternate Names |
|
| Background Information | ASF1a (anti-silencing function 1a) is a histone H3/H4 chaperone and chromatin regulator. This protein facilitates histone placement and histone removal and exchange during nucleosome assembly/disassembly and is essential for the formation of senescence-associated heterochromatic foci (SAHF). ASF1a interacts with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA inside PML bodies of cells approaching senescence after p53/p21 activation. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-ASF1a Antibody is a Rabbit Polyclonal Antibody for detection of ASF1a also known as Histone chaperone ASF1A or hAsf1 & has been validated in WB & IP. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Banumathy, g, et Al. (2009). Mol Cell Biol. 29(3):758–770.) |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human ASF1a. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. SUBUNIT STRUCTURE: Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1. SUBCELLULAR LOCATION: Nucleus TISSUE SPECIFICTY: Ubiquitously expressed. PTM: Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase. SEQUENCE SIMILARITIES: Belongs to the ASF1 family. |
| Molecular Weight | ~23 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected ASF1a on 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |