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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, Chp, Rhesus Macaque | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE198 |
| Description | Anti-Sirt5 Antibody |
| Alternate Names |
|
| Background Information | Sirtuin (SIRT5) is known as a nicotinamide adenine dinucleotide- dependent deacetylase that is a member of the silent information regulator 2 (Sir2) family of proteins that is important in aging, metabolism and disease. SIRT5 is expressed in a variety of tissues. Two different isoform have been described. Isoform 1 is reported to be express in the mitochondria and cytoplasm while isoform 2 appears to be localized to the mitochondria only. SIRT5 has also been linked to many primate-specific functions. SIRT5 has recently been implicated as having a role in adaptation to fasting, caloric restriction and high protein diets. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-Sirt5 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect Sirt5 also known as NAD-dependent deacetylase sirtuin-5, SIR2-like protein 5. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: 1:500 dilution of a representative lot of this antibody detected Sirt5 in convoluted tubules of normal rat kidney tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Sirt5. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human and Rat. Predicted to react with Chimpanzee and Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: NAD-dependent protein deacetylase that activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting. Deacetylates CPS1 and increases CPS1 activity in response to elevated NAD levels during fasting (by similarity). Can deacetylate cytochrome c and a number of other proteins (in vitro). CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. COFACTOR: Binds 1 zinc ion per subunit. ENZYME REGULATION: Inhibited by suramin. SUBUNIT STRUCTURE: Monomer. Homodimer. Forms homodimers upon suramin binding. Interacts with CPS1 (by similarity). SUBCELLULAR LOCATION: Mitochondrion matrix. Mitochondrion intermembrane space (potential). TISSUE SPECIFICITY: Widely expressed. SEQUENCE SIMILARITIES: Belongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain. |
| Molecular Weight | ~34 kDa observed. Two isoforms at ~33 and ~34 kDa are known to exist. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Sirt5 on 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |