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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE251 |
| Replaces | 06-892 |
| Description | Anti-N-CoR Antibody |
| Alternate Names |
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| Background Information | In the absence of ligands, the corepressor N-CoR represses nuclear hormone receptor-mediated gene transcription. In the presence of ligands, these hormone receptors activate gene expression and play critical roles in development, growth, and homeostasis. N-Cor-mediated transcriptional repression is facilitated through the formation of histone deacetylase (HDACs) N-CoR complexes or by conjugation from small ubiquitin-like modifier 1 (SUMO-1). It is shown that the SANT1 domain of N-CoR serves as a binding region for SUMO-E2 conjugating enzyme Ubc9 and SUMO-E3 ligase Pias1, which is necessary for N-CoR-mediated repression of respective reporter genes. SUMOylation-deficient N-COR knockout mice display a signification reduction in the repression of gene transcription and result in defects involving the differentiation and development of neural cells into erythrocytes and thymocytes. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-N-CoR Antibody is a Rabbit Polyclonal Antibody for detection of N-CoR also known as Nuclear receptor corepressor 1 & has been validated in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a region between amino acids 1510 and 1540 of the N-CoR protein. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. SUBUNIT STRUCTURE: Interacts with ZBTB33; the interaction serves to recruit the N-CoR complex to promoter regions containing methylated CpG dinucleotides. Interacts with TRIM28 and KDM3A. Interacts (via the RD1 domain) with BAZ1A (via its N-terminal); the interaction corepresses a number of NCOR1-regulated genes. Interacts with HEXIM1 By similarity. Interacts with C1D, SIAH2, HDAC7, SAP30, SIN3A and SIN3B. Forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid receptor (TR) and the retinoid acid receptor (RAR) in the absence of ligand. Interacts directly with RARA; the interaction is facilitated with RARA trimethylation. Interacts with DACH1. Component of the N-Cor repressor complex, at least composed of CBFA2T3, HEXIM1, NCOR1, NCOR2, HDAC3, TBL1X, TBL1XR1, CORO2A and GPS2. SUBCELLULAR LOCATION: Nucleus. |
| Molecular Weight | ~270 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at MW of 266 kDa and 270 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in RAW 264.7 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected N-CoR in RAW 264.7 cell lysate. No tween for best results. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |