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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M, B | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN34 |
| Description | Anti-PDE11A Antibody |
| Alternate Names |
|
| Background Information | The PDE11A gene encodes for a dual-specificity Phosphodiesterase (PDE), which is expressed in the adrenal cortex and is partially inhibited by tadalafil and other PDE inhibitors. PDEs are known to catalyze cAMP and cGMP to 5’-AMP and 5’GMP, respectively and thereby regulate the intracellular concentration of these nucleotides. Loss-of-function mutation of the PDE11A gene is shown to increase cAMP signaling and cAMP-responsive element binding protein (CREB) phosphorylation, two conditions which are associated with adrenocortical hyperplasia, adenomas, and Cushing syndrome. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect PDE11A using this Anti-PDE11A Antibody validated for use in WB, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PDE11A in normal mouse midbrain tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the GAF domain of human PDE11A. |
| Epitope | GAF domain. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes GAF domain of PDE11A. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human and Mouse. Predicted to react with Rat, and Bovine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The 3,5-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3,5-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a member of the PDE protein superfamily. Mutations in this gene are a cause of Cushing disease and adrenocortical hyperplasia. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5-AMP and 5-GMP, respectively. CATALYTIC ACTIVITY: Guanosine 3,5-cyclic phosphate + H2O = guanosine 5-phosphate. Adenosine 3,5-cyclic phosphate + H2O = adenosine 5-phosphate. COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. ENZYME REGULATION: Inhibited by 3-isobutyl-1-methylxanthine (IBMX), zaprinast and dipyridamole. cGMP acts as an allosteric activator. Weakly inhibited by Sildenafil (Viagra) and Tadalafil (Cialis); however, the fact that the protein is probably absent from testis, suggests that it is not biologically relevant and is not related with erectile dysfunction. SUBCELLULAR LOCATION: Cytoplasm > cytosol. TISSUE SPECIFICITY: Isoform 1 is present in prostate, pituitary, heart and liver. It is however not present in testis nor in penis, suggesting that weak inhibition by Tadalafil (Cialis) is not relevant (at protein level). Isoform 2 may be expressed in testis. Isoform 4 is expressed in adrenal cortex. |
| Molecular Weight | ~104 kDa observed. Uniprot states four isoforms at a molecular weight of ~105 kDa, ~78 kDa, ~66 kDa, and ~56 kDa. This antibody can only recognize the ~105 kDa and the ~78 kDa isoforms. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human hippocampus tissue lysate. Western Blot Analysis: 1 µg/mL of this antibody detected PDE11A in 10 µg of human hippocampus tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |