Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN40 |
| Description | Anti-DLK1 Antibody |
| Alternate Names |
|
| Background Information | Delta-like 1 homolog (DLK1), also known as fetal antigen 1 (FA1) and preadipocyte factor 1 (pref-1), is a transmembrane protein containing 6 epidermal growth factor repeats whose expression is known to modulate differentiation signals. Studies have shown DLK1 is involved in differentiation of several cell types, including adipocytes. Studies have shown deficiencies in DLK1 displaying growth retardation, obesity, skeletal malformation, and increased serum lipid. DLK1 is a paternally expressed imprinted gene that plays a role in normal development and in the maintence of homeostatsis of adipose tissue mass. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-DLK1 Antibody is validated for use in WB, IH for the detection of DLK1. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected DLK1 in mouse embryonic tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human DLK1. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. [provided by RefSeq, Mar 2010]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | SUBUNIT STRUCTURE: Monomer. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. PTM: N- and O-glycosylated. SEQUENCE SIMILARITIES: Contains 6 EGF-like domains. SEQUENCE CAUTION: The sequence CAA35582.1 differs from that shown. Reason: Frameshift at positions 22, 108 and 199. |
| Molecular Weight | ~47 kDa observed. The calculated molecular weight of this protein is 41 kDa, but can be observed at ~41-55 kDa due to high glycosylation. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HepG2 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected DLK1 on 10 µg of HepG2 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |