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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IHC, IF | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABD76 |
| Description | Anti-Oct-4 Antibody, clone 9B7 |
| Alternate Names |
|
| Background Information | The POU5F1 gene expresses the POU-domain transcription factor, Octamer-4 (Oct-4), which is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Oct-4 Antibody, clone 9B7 detects level of Oct-4 & has been published & validated for use in WB, IH & IF. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in human lung tissue. Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in COS7 cells. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human Oct-4. |
| Clone | 9B7 |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Mouse. Predicted to react with Human based on immunogen design. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewings sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Transcription factor that binds to the octamer motif (5-ATTTGCAT-3). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. SUBUNIT STRUCTURE: Interacts with UBE2I and ZSCAN10 By similarity. Interacts with PKM2. Interacts with WWP2. SUBCELLULAR LOCATION: Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern. TISSUE SPECIFICITY: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. |
| Molecular Weight | ~39 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse embryonic stem cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Oct-4 in 10 µg of mouse embryonic stem cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |