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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN125 |
| Description | Anti-DRD4 Antibody, clone 2B9 |
| Alternate Names |
|
| Background Information | Five subtypes of dopamine receptors exist in two subfamilies: two D1-like (D1 and D5) and three D2-like (D2, D3 and D4). As members of the G protein-coupled receptor (GPCR) family, dopamine receptors initiate their biological function by coupling to GTP regulatory proteins (G proteins). D1-like receptors, DRD1 and DRD5, stimulate adenylyl cyclase whereas D2-like receptors, DRD2, DRD3 and DRD4, are coupled to inhibitory G proteins and their stimulation leads to the inhibition of adenylyl cyclase activity, and subsequent respective cellular signaling. All five subtypes have seven transmembrane domains, an extracellular amino terminus, and an intracellular carboxyl terminus. Allelic variants of the D4 dopamine receptor (DRD4) are known characterized risk factors of ADHD. It is reported that hypoxia enhances DRD4 promoter activity by several fold. It is also thought that dopamine receptors such as DRD4 are involved in neuroexcitation and in a variety of other neurological and psychiatric disorders. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-DRD4 Antibody, clone 2B9 is validated for use in WB, IC for the detection of DRD4. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 0.5 µg/mL from a representative lot detected DRD4 in 10µg of rat brain tissue lysate. Immunocytochemistry Analysis: A representative lot from an independent laboratory detected DRD4 in Ntera-2 cells and in rat brain neurons. (Wolstencroft, E.C., et al. (2007). J Mol Histol. 38(4):333-340.) |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to human DRD4. |
| Clone | 2B9 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. SUBUNIT STRUCTURE: Interacts with CLIC6 and GPRASP1. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. |
| Molecular Weight | ~ 48 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse brain tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected DRD4 in 10µg of mouse brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |