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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN92 |
| Description | Anti-Iba1/AIF1 Antibody |
| Alternate Names |
|
| Background Information | Allograft inflammatory factor 1 (UniProt: P55008; also known as AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, Iba1) is encoded by the AIF1 (also known as G1, IBA1) gene (Gene ID: 199) in human. Iba1/AIF1 is a highly conserved cytoplasmic, calcium binding, inflammation responsive scaffold protein that is detected in T-lymphocytes and peripheral blood mononuclear cells. It has also been shown to associate with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. It is not present in normal arteries, but its expression is induced in vascular smooth muscle cells in response to arterial injury where it enhances growth and promote proliferation in vascular smooth muscle cells through deregulation of the cell cycle by shortening the cell cycle and altering the expression of cyclins. AIF1 is also found in activated macrophages where its expression is augmented by cytokines, particularly interferon gamma. It is also considered as a marker for activated macrophages. Serum AIF-1 levels have been correlated with albuminuria and eGFR in type 2 diabetic subjects and serves as a marker for diabetic nephropathy. Three isoforms of AIF1/IBA1 have been described that are produced by alternative splicing. This clone will react with isoforms 1 and 2 that contain this immunogen sequence. (Ref.: Fukui, M., et Al. (2012). Diabetes Res. Clin. Pract. 98(1); 146-150; Autieri, MV., and Carbone, cm (2001). Arterioscler. Thromb. Vasc. Biol. 21(9);1421-1426). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Iba1/AIF1 Antibody is an antibody against Iba1/AIF1 for use in WB, IH. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 1 µg/mL from a representative lot detected Iba1/AIF1 in 10 µg of mouse thymus tissue lysate. Western Blot Analysis: 1 µg/mL from a representative lot detected Iba1/AIF1 in 10 µg of rat thymus tissue lysate. Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected Iba/AIF1 in human spleen tissue. Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Iba/AIF1 in rat spleen tissue |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Iba1/AIF1. |
| Clone | 20A12.1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. SUBUNIT STRUCTURE: Homodimer. Monomer. Interacts with LCP1. SUBCELLULAR LOCATION: Cytoplasm > cytoskeleton. Cell projection > ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. TISSUE SPECIFICITY: Detected in T-lymphocytes and peripheral blood mononuclear cells. |
| Molecular Weight | ~17 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated in Western Blot in human thymus tissue lysate. Western Blot Analysis: 1 µg/mL of this antibody detected Iba1/AIF1 in 10 µg of human thymus tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |