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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC, ELISA, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT64 |
| Description | Anti-Nectin-4/PVRL4 Antibody, clone N4.61 |
| Alternate Names |
|
| Background Information | Nectin-4 is also known as poliovirus receptor-related protein 4 (PRR4 or PVRL4). Nectins are known for being the receptors for the herpes simplex virus. Nectin-4 is an immunoglobulin-like transmembrane cell adhesion protein that also seems to play a role in neural tube formation, with N-cadherin. Nectin-4 is connected with the actin cytoskeleton through afadin inside the cell. Nectin-4 is predominantly found in human placenta although in mice, expression is wide-ranging. Nectin-4 seems to adhere primarily to cells expressing nectin-1 and vice versa. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Nectin-4/PVRL4 Antibody, clone N4.61 is an antibody against Nectin-4/PVRL4 for use in IH, ELISA & IP. |
| Key Applications |
|
| Application Notes | ELISA Anaysis: A previous lot was used by an independent laboratory in ELISA (Fabre-Lafay, S., et al. (2005). Journal of Biological Chemistry. 280(20):19543–19550). Immunoprecipitation Anaysis: A previous lot was used by an independent laboratory in IP (Fabre-Lafay, S., et al. (2005). Journal of Biological Chemistry. 280(20):19543–19550). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human Nectin-4. |
| Clone | N4.61.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Poliovirus receptor-like proteins (PVRLs), such as PVRL4, are adhesion receptors of the immunoglobulin superfamily and function in cell-cell adhesion (Reymond et al., 2001 [PubMed 11544254]).[supplied by OMIM]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells. SUBUNIT STRUCTURE: Self-associates. Interacts via its Ig-like V-type domain with PVRL1/nectin-1 Ig-like V-type domain. Interacts via its C-terminus with MLLT4. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein Potential. Cell junction › adherens junction. Note: Colocalizes with MLLT4 at cadherin-based adherens junctions. Processed poliovirus receptor-related protein 4: Secreted. Note: The secreted form is found in breast tumor patients. TISSUE SPECIFICTY: Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma. PTM: The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE. SEQUENCE SIMILARITIES: Belongs to the nectin family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| Molecular Weight | 55 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in ductal carcinoma tissue. Immunohistochemistry Analysis: A 1:400 dilution of this antibody detected Nectin-4/PVRL4 in ductal carcinoma tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |