Restek Alkylation Derivatization Reagent, TMPAH, 10x1 g Vials, 10-pk.

Alkylation reagents reduce molecular polarity by replacing active hydrogens, such as carboxylic acids and phenols. Alkylation reagents can be used alone to form esters and amides, or they can be used in conjunction with acylation or silylation reagents. A two-step approach is commonly used in the derivatization of amino acids, where multiple functional groups of these compounds may necessitate protection during derivatization.

Esterification—the most popular method of alkylation due to the availability of reagents and ease of use—is the reaction of an acid with an alcohol in the presence of a catalyst. Alkyl esters are stable and can be formed quickly and quantitatively. Retention of the derivative can be varied by altering the length of the substituted alkyl group. In addition to the formation of simple esters, alkylation reagents can be used in extraction procedures where biological matrices are present.