Takara Sialic Acid Fluorescence Labeling Kit \100 Rxns

The fluorescence labeling of sialic acid with 1, 2-diamino-4, 5-methyleneoxybenzene (DMB) is a simple and very sensitive analytical method in which free sialic acids are analyzed by reverse-phase HPLC after labeling with DMB. The sialic acids which are bound to the sugar chains in glycoproteins or glycolipids can also be measured after pre-treatment with sialidases or acid hydrolysis. The detection limit of DMB-sialic acid is 57 fmol. TaKaRas Sialic Acid Fluorescence Labeling Kit is designed to simplify the DMB-derivatization procedure. The reagents are provided in aqueous solutions and the protocol only involves mixing of reagents and incubation. By combination of this DMB method with Pyridylamino (PA) derivatization, sialoglycoconjugates can be analyzed with extremely high sensitivity.