Takara SMART-Seq® Total RNA Pico Input (ZapR™ Mammalian) \ 24 Rxns

SMART-Seq Total RNA Library Prep with ZapR Depletion generates strand-specific RNA-seq libraries for Illumina sequencing from 250 pg–1 µg inputs of purified total RNA. The protocol for use with this kit was developed to work with either high- or low-quality total RNA, does not require additional rRNA removal methods or kits, and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNA—typically present in high abundance following cDNA synthesis from total RNA inputs—makes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA. Enough material is provided with this kit to perform up to 24 reactions.

Efficient depletion of rRNA-derived cDNAs across a wide input range.SMART-Seq Total RNA Library Prep was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Library Prep with ZapR Depletion) and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 2 x 106paired-end reads. The bar graph (Panel A) and table (Panel B) show the read distribution of 250 pg-input and 10 ng-input libraries treated with the ZapR Mammalian rRNA Depletion Kit compared to 250 pg-input untreated libraries.

Superior gene and transcript detection across a wide input range.SMART-Seq Total RNA Library Prep was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Library Prep with ZapR Depletion) and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 2 x 106paired-end reads. Gene counts (Panel A) and gene and transcript counts (Panel B) are increased for ZapR-treated libraries.

Preservation of gene abundance information across a wide input range. SMART-Seq Total RNA Library Prep with ZapR Depletion was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Data analysis was performed with CogentAP using 2 x 106paired-end reads. The scatterplot illustrates the high correlation between the 10 ng-input libraries and 250 pg-input libraries.

Required Products

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.