Key Specifications Table
| Key Applications |
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| Cell Based Assays |
| Description | |
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| Catalogue Number | SCT044 |
| Description | BioTracker LYSO-TP Live Cell Dye |
| Overview | Hypochlorous acid (HOCl) is a highly potent reactive oxygen species (ROS) and helps eliminate pathogens in the innate immune system. Mounting evidence indicates that intracellular HOCl plays additional important roles in regulating inflammation and cellular apoptosis. Subcellular detection of HOCL is currently limited due to low concentration, strong oxidization, and short lifespan of the analyte. The BioTracker LYSO-TP dye is a live cell two-photon green fluorescent “turn-on” imaging probe for HOCL. The probe targets HOCL specifically in the lysosomes of cells. The probe exhibit fast response times, good selectivity, and high sensitivity towards hypochlorous acid in living cells. Acedan was chosen as the fluorescence reporting group due to its excellent photophysical properties resulting from the typical “push−pull” (amineketone) structure. Reaction of the probe with HOCl, which deprotects the oxathiolane/mercaptal group to reveal the ketone, would lead to fluorescence enhancement. To monitor subcellular HOCL levels, morpholine was introduced at a long distance from the reaction site to ensure the probe’s intracellular localization. Spectral Properties Absorbance: 375nm Emission: 500nm References Chang YT et al. Development of Targetable Two-Photon Fluorescent Probes to Image Hypochlorous Acid in Mitochondria and Lysosome in Live Cell and Inflamed Mouse. Model. J Am Chem Soc. 2015 May 13;137(18):5930-8. |
| Alternate Names |
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| Product Information | |
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| Detection method | Fluorescence |
| Presentation | Lyophilized |
| Applications | |
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| Application | Live cell imaging dye for hypochlorous acid (HOCl) targeted to cellular lysosomes used to detect phagocytosis, inflammation and cellular apoptosis. |
| Key Applications |
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| Application Notes | Live cell fluorescent imaging |
| Product Usage Statements | |
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| Quality Assurance | Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis Molar Mass: 357.49 g/mol |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Store BioTracker LYSO-TP Live Cell Dye at -20°C, desiccate and protect from light Note: Centrifuge vial briefly to collect contents at bottom of vial before opening. |
| Packaging Information | |
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| Material Size | 1 mg |