Takara Chaperone Plasmid Set \1 Set

The Chaperone Plasmid Set consists of five different plasmids, each of which is designed to express multiple molecular chaperones that function as a "chaperone team" to enable protein folding. Co-expression of a target protein with one of these chaperone teams increases the recovery of soluble proteins. Each plasmid carries an origin of replication derived from pACYC and a Cmrgene, which allows use withE. coliexpression systems utilizing ColE1-type plasmids containing an ampicillin resistance gene as a marker. The chaperone genes are situated downstream of anaraBorPzt-1(tet) promoter. Therefore, expression of target proteins and chaperones can be induced individually if the target gene is placed under the control of other promoters (e.g.lac). These plasmids also contain the necessary regulator (araCortetr) for each promoter.

Use this set to perform a two step method to construct target/chaperone co-expression systems: In the first step prepare anE. colihost transformed with only the chaperone plasmid. The second step is to prepare competent cells from this new strain and transform this strain with a plasmid expressing the target protein.

NOTE:For commercial use of this product, please refer to theprotein expression vectors for commercial use pagefor ordering information.

Vector maps of Takara Bios chaperone plasmids.