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GenScript Cho-K1/Cd32b Ile232 Stable Cell Line ; 2vials
List Price
$9,371.25
Your Price
$9,371.25
GenScript Cho-K1/Cd32b Ile232 Stable Cell Line 2vials - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-M00587
MFG.PART:
M00587
UNSPSC:
41106514
Manufacturer:
GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cells stably overexpress low affinity immunoglobulin gamma Fc region receptor II-b (FcγRIIb / CD32B 232Ile). The surface expression of CD32B 232Ile is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of antibody based biologics binding with CD32B 232Ile. GenScript offers both CD32B 232Ile expressing stable cell line and CD32B 232Thr expressing stable cell line (Cat. No. M00600) for FcγRIIb polymorphism study. |
| Applications | Cell-based binding assay; use as immunogen for antibody development |
Product Introduction
| Expressed Gene | Codon optimized from NM_004001.4; no expressed tags |
| Target Protein | NP_003992.3 |
| Host Cell | CHO-K1 |
| Quantity | 2 vials of frozen cells (>1X106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | F-12K (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium | F-12K, 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Gibco) |
Background
| Gene Synonyms | FCGR2B; FCG2; IGFR2; CD32 |
| Background | Human CD32 group consists of FcγRIIa, b and c proteins that share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32B, also known as FcγRIIb, is a low affinity receptor that recognizes immunoglobulin gamma complexes. It is encoded by the FCGR2B gene and expressed on B cells and most myeloid cells. CD32B has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Co-ligation of CD32B with B cells antigen receptor (BCR) results in selective dephosphorylation of CD19 tyrosine, tyrosine phosphorylation of the ITIM in the CD32B cytoplasmic domain, and recruitment of SH2-containing inositol 5-phosphatase (SHIP). This causes a block in calcium influx, inhibition of B cell activation, and leads to apoptosis. CD32B is a potential target for monoclonal antibody therapy for malignancies. Targeting CD32B may represent a novel approach to immunotherapy of B-cell malignancies. |
| SKU | GSCRPT-M00587 |
|---|---|
| Featured | No |
| Supplier Part Number | M00587 |
| UM | EA |
| UNSPSC | 41106514 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |