We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn about our cookie policy.
GenScript Cho-K1/Cd64 Stable Cell Line; 2vials
List Price
$9,371.25
Your Price
$9,371.25
GenScript Cho-K1/Cd64 Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-M00588
MFG.PART:
M00588
UNSPSC:
41106514
Manufacturer:
GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cellsstably overexpress high affinity human Fc fragment of IgG receptor Ia (FcγRI,CD64) on the cell surface, containingone alpha chain and two gamma chains. The surface expression of CD64 isvalidated by FACS analysis. This stable cell line product is designed for measuringthe binding affinity and stability of the Fc region of antibodies, Fc fusionproteins, or antibody based biologics that bind with CD64 during biologics research and development. |
| Applications | Cell-based binding assay; use as immunogen for antibodydevelopment |
Product Introduction
| Expressed Gene | Codon Optimized from NM_000566.3 (for FCGR1A); CodonOptimized from NM_004106.2 (for FCER1G); No expressed tags. |
| Host Cell | CHO-K1 |
| Quantity | 2 vialsof frozen cells (>1×106 per vial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately uponreceipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative.The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit(Cat. No. LT07-318, Lonza). |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% complete growth medium,5% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | Hams F-12K (Kaighns) (Cat.No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco) |
| Culture Medium | Hams F-12K (Kaighns) (Cat. No.21127-022, Gibco), 10%FBS (Cat. No.10099-141, Gibco), 300µg/ml Hygromycin B (Cat. No.10687010, Invitrogen), 8 μg/ml Puromycin (Cat.No.A11138-03, Gibco) |
Background
| Gene Synonyms | FCRI, CD64A, FCGR1, FCGR1A (for Fc Fragment of IgGreceptor Ia); FCER1G, FCRG (for Fc fragment of IgE receptor Ig) |
| Background | CD64(Cluster of Differentiation 64) is a type of integral membrane glycoprotein, alsoknown as Fc-gamma receptor 1 (FcγRI). It is composed of two subunits- an alphasubunit encoded by FCGR1A (Fc Fragment of IgG Receptor Ia), and a gamma subunitencoded by FCER1G (Fc Fragment of IgE Receptor Ig). It binds monomeric IgG-typeantibodies with high affinity. After binding IgG, CD64 interacts with anaccessory chain known as the common γ chain (γ chain), which possesses an ITAMmotif that is necessary for triggering cellular activation. While CD64 isconstitutively found only on macrophages and monocytes, treatment ofpolymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64expression on these cells. |
| SKU | GSCRPT-M00588 |
|---|---|
| Supplier Part Number | M00588 |
| UM | EA |
| UNSPSC | 41106514 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately uponreceipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |