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GenScript Cho-K1/Human Cd32a 131his Stable Cell Line; 2vials
List Price
$9,371.25
Your Price
$9,371.25
GenScript Cho-K1/Human Cd32a 131his Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-M00598
MFG.PART:
M00598
UNSPSC:
41106514
Manufacturer:
GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cellsstably overexpress human immunoglobulin gamma Fc region receptor II-a(FcγRIIa / CD32A 131His). The surfaceexpression of CD32A 131His is validated by FACS analysis. This stable cell lineproduct is designed for measuring binding affinity and stability of Fc regionof antibodies and Fc fusion proteins, or antibody based biologics binding withCD32A 131His. GenScript also offers CD32A131Arg stable cell line (Cat. No. M00887) for FcγRIIapolymorphism study. |
| Applications | Cell-based binding assay; use as immunogen for antibodydevelopment |
Product Introduction
| Expressed Gene | Codon optimized from NM_001136219.1; no expressed tags |
| Target Protein | NP_001129691.1 |
| Host Cell | CHO-K1 |
| Quantity | Twovials of frozen cells (>1×106 per vial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately uponreceipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative.The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit(Cat. No. LT07-318, Lonza). |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% complete growth medium,5% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s)(Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10%FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco) |
Background
| Gene Synonyms | CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1 |
| Background | CD32is a surface receptor protein and part of a large population of B cellco-receptors, which act to modulate signaling. It has a low-affinity for IgGantibodies and down-regulates antibody production in the presence of IgG. Thisfeedback loop acts to lower the production of IgG by B cells when there is asurplus in the body. Monoclonal antibodies can distinguish between CD32A andCD32B. |
| SKU | GSCRPT-M00598 |
|---|---|
| Supplier Part Number | M00598 |
| UM | EA |
| UNSPSC | 41106514 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |