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GenScript Cho-K1/Cd32b 232thr Stable Cell Line; 2vials
List Price
$9,371.25
Your Price
$9,371.25
GenScript Cho-K1/Cd32b 232thr Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-M00600
MFG.PART:
M00600
UNSPSC:
41106514
Manufacturer:
GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cells stably overexpress low affinity immunoglobulin gamma Fc region receptor II-b (FcγRIIb / CD32B 232Thr). The surface expression of CD32B 232Thr is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD32B 232Ile. GenScript also offers CD32B 232Ile overexpression stable cell line (Cat. No. M00587) for FcγRIIb polymorphism study. |
| Applications | Cell-based binding assay; use as immunogen for antibody development |
Product Introduction
| Expressed Gene | Codon optimized from KR710627.1; no expressed tags |
| Target Protein | AKI71009.1 |
| Host Cell | CHO-K1 |
| Quantity | 2 vials of frozen cells (>1×106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | Hams F-12K (Kaighns) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco) |
| Culture Medium | Hams F-12K (Kaighns) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No.A11138-03, Gibco) |
Background
| Gene Synonyms | CD32; FCG2; CD32B; FCGR2; IGFR2 |
| Background | FCGR2B (CD32B) is a low affinity receptor for Immunoglobulin G (IgG). Mutation in the gene in humans leads to a lupus phenotype. The cytoplasmic part of this receptor contains an immunoreceptor tyrosine-based inhibitory motif, in contrast to the activating isoform, FCGR2A (CD32A). |
| SKU | GSCRPT-M00600 |
|---|---|
| Featured | No |
| Supplier Part Number | M00600 |
| UM | EA |
| UNSPSC | 41106514 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |