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GenScript Cho-K1/Cd32c 13gln Stable Cell Line; 2vials
List Price
$9,371.25
Your Price
$9,371.25
GenScript Cho-K1/Cd32c 13gln Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-M00601
MFG.PART:
M00601
UNSPSC:
41106514
Manufacturer:
GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cellsstably overexpress Homo sapienscell-type natural killer cells Fc gamma receptor IIc1 (FcγRIIc/ CD32C) on the cell surface. The surface expression of FcγRIIcis validated by FACS analysis. This stable cell line is recommended forevaluating the binding affinity between FcγRIIcand Fc region of antibodies, Fc fusion proteins, or antibody-based biologics. |
| Applications | Cell-based binding assay; use as immunogen forantibody development |
Product Introduction
| Expressed Gene | Codonoptimized from U90938.1; no expressed tags |
| Target Protein | AAC12807.1 |
| Host Cell | CHO-K1 |
| Size | Two vials of frozen cells (>1×106 pervial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately uponreceipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative.The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit(Cat. No. LT07-318, Lonza). |
| Stability | Stable through more than 15 passages with nosignificant changes in assay performance or expression profile. |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% Complete Growth Medium, 5% DMSO (Cat. No. D2650,Sigma) |
| Complete Growth Medium | Hams F-12K (Kaighns) Medium (Cat. No. 21127-022,Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium | Hams F-12K (Kaighns) Medium (Cat. No. 21127-022,Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03,Gibco) |
Background
| Gene Synonyms | CD32;FCG2; CD32C; CDW32; IGFR2; FCRIIC |
| Background | Human studies suggestexpression of CD32C could help predict patient responses to vaccines. CD32Cnormally contains a termination codon, but 7%–15% of individuals carry amutation that changes it into a full-length open reading frame. Individualswith this variant expressed CD32C on B cells and were more likely to respond toan anthrax vaccine than patients that did not express the variant. Individualswith the full-length CD32C variant also were more likely to develop theautoimmune disease systemic lupus erythematosus (SLE) than those without thevariant. |
| SKU | GSCRPT-M00601 |
|---|---|
| Supplier Part Number | M00601 |
| UM | EA |
| UNSPSC | 41106514 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately uponreceipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |