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GenScript Cho-K1/Cd32c 13gln Stable Cell Line; 2vials
List Price
$9,371.25
Your Price
$9,371.25
GenScript Cho-K1/Cd32c 13gln Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-M00601
MFG.PART:
M00601
UNSPSC:
41106514
Manufacturer:
GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cells stably overexpress Homo sapiens cell-type natural killer cells Fc gamma receptor IIc1 (FcγRIIc / CD32C) on the cell surface. The surface expression of FcγRIIc is validated by FACS analysis. This stable cell line is recommended for evaluating the binding affinity between FcγRIIc and Fc region of antibodies, Fc fusion proteins, or antibody-based biologics. |
| Applications | Cell-based binding assay; use as immunogen for antibody development |
Product Introduction
| Expressed Gene | Codon optimized from U90938.1; no expressed tags |
| Target Protein | AAC12807.1 |
| Host Cell | CHO-K1 |
| Size | Two vials of frozen cells (>1×106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability | Stable through more than 15 passages with no significant changes in assay performance or expression profile. |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% Complete Growth Medium, 5% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | Hams F-12K (Kaighns) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium | Hams F-12K (Kaighns) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco) |
Background
| Gene Synonyms | CD32; FCG2; CD32C; CDW32; IGFR2; FCRIIC |
| Background | Human studies suggest expression of CD32C could help predict patient responses to vaccines. CD32C normally contains a termination codon, but 7%–15% of individuals carry a mutation that changes it into a full-length open reading frame. Individuals with this variant expressed CD32C on B cells and were more likely to respond to an anthrax vaccine than patients that did not express the variant. Individuals with the full-length CD32C variant also were more likely to develop the autoimmune disease systemic lupus erythematosus (SLE) than those without the variant. |
| SKU | GSCRPT-M00601 |
|---|---|
| Featured | No |
| Supplier Part Number | M00601 |
| UM | EA |
| UNSPSC | 41106514 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |