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GenScript Cho-K1/Cd16b Na1 Stable Cell Line; 2vials
List Price
$9,371.25
Your Price
$9,371.25
GenScript Cho-K1/Cd16b Na1 Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-M00602
MFG.PART:
M00602
UNSPSC:
41106514
Manufacturer:
GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cells stably overexpress human Fc fragment of IgG receptor IIIb (FcγRIIIb / CD16B NA1). The surface expression of CD16B NA1 is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD16B during biologics research and development. |
| Applications | Cell-based binding assay; use as immunogen for antibody development |
Product Introduction
| Expressed Gene | Codon optimized from CCDS41433.1; no expressed tags |
| Target Protein | NP_000561.3 |
| Host Cell | CHO-K1 |
| Quantity | 2 vials of frozen cells (>1×106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | Hams F-12K (Kaighns) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco) |
| Culture Medium | Hams F-12K (Kaighns) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco) |
Background
| Gene Synonyms | CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb |
| Background | CD16 has been identified as FcγRIIIa (CD16A) and FcγRIIIb (CD16B) which are similar to each other. CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells. CD16B exists as a monomeric glycosylphosphatidylinositol (GPI)-anchored form in neutrophils [1]. CD16B (FcγRIIIb) exists in two allelic forms, NA1 and NA2, [2-3]. The polymorphism of CD16B exhibits different binding affinities for IgG1 and IgG3 subclasses [3]. The different allelic versions are also demonstrated to be associated with susceptibility to diseases like idiopathic pulmonary fibrosis [4] and systemic lupus [5]. |
| SKU | GSCRPT-M00602 |
|---|---|
| Featured | No |
| Supplier Part Number | M00602 |
| UM | EA |
| UNSPSC | 41106514 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |