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GenScript Cho-K1/Fcrn Stable Cell Line; 2vials
List Price
$9,371.25
Your Price
$9,371.25
GenScript Cho-K1/Fcrn Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-M00603
MFG.PART:
M00603
UNSPSC:
41106514
Manufacturer:
GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cells stably overexpress human FcRn and B2M subunits. The B2M subunits enable stable expression of FcRn in cells. Association of FcRn alpha-chain with beta (2) m is also important for IgG binding. The surface expression of FcRn is validated by FACS analysis. This cell can be used in binding assay or as an immunogen. |
| Applications | Binding assay or use as immunogen |
Product Introduction
| Expressed Gene | Codon optimized from NM_004107 (for FcRn); AF072097 (for B2M); no expressed tags |
| Target Protein | NP_004098.1 (for FcRn), AAD48083.1 (for B2M) |
| Host Cell | CHO-K1 |
| Size | Two vials of frozen cells (1×106 per vial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability | Stable through more than 15 passages without significant changes in assay performance or expression profile. |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. A5669701, Gibco) |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Gibco), 200 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen) |
Background
| Gene Synonyms | FCGRT, alpha-chain, FcgammaRn (for FcRn), and Beta-2-Microglobulin (for B2M) |
| Background | This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation. |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
| SKU | GSCRPT-M00603 |
|---|---|
| Featured | No |
| Supplier Part Number | M00603 |
| UM | EA |
| UNSPSC | 41106514 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |