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GenScript Cho-K1/Cd32a 131arg Stable Cell Line ; 2vials
List Price
$9,371.25
Your Price
$9,371.25
GenScript Cho-K1/Cd32a 131arg Stable Cell Line 2vials - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-M00887
MFG.PART:
M00887
UNSPSC:
41106514
Manufacturer:
GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cells stably overexpress immunoglobulin gamma Fc region receptor II-a (FcγRIIa / CD32A 131Arg). The surface expression of CD32A 131Arg is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of antibody based biologics binding with CD32A 131Arg. GenScript offers both CD32A 131Arg stable cell line and CD32A 131His stable cell line (Cat. No. M00589) for FcγRIIa polymorphism study. |
| Applications | Cell-based binding assay; use as immunogen for antibody development |
Product Introduction
| Expressed Gene | Codon optimized from NM_001136219.1; no expressed tags |
| Target Protein | NP_001129691.1 |
| Host Cell | CHO-K1 |
| Quantity | 2 vials of frozen cells (>1X106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | F-12K (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium | F-12K, 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco) |
Background
| Gene Synonyms | CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1 |
| Background | Human CD32 group consists of FcγRIIA, B and C proteins that share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32A, also known as FcγRIIA, is a surface receptor protein and part of a large population of B cell co-receptors. The protein is found on phagocytic cells such as macrophages and neutrophils, and it is involved in the process of phagocytosis and clearing of immune complexes. It has low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loop acts to lower the production of IgG by B cells when there is a surplus in the body. Therefore, targeting CD32A may represent an approach to immunotherapy of B-cell malignancies. |
| SKU | GSCRPT-M00887 |
|---|---|
| Featured | No |
| Supplier Part Number | M00887 |
| UM | EA |
| UNSPSC | 41106514 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |