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Millipore Anti-Rac1 Antibody, Clone 23a8, Alexa Fluor® 647 Conjugate Is An Antibody Against Rac1 For Use In Immunocytochemistry. - Mill (Additional S&H or Hazmat Fees May Apply)
Ras-related C3 botulinum toxin substrate 1 (UniProt P63000; also known as Cell migration-inducing gene 5 protein, p21-Rac1, Rac-1, Ras-like protein TC25, TC-25) is encoded by the RAC1 (also known as TC25) gene (ORF MIG5; Gene ID 5879) in human. Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Akt is reported to negatively regulate mixed-lineage kinase 3 (MLK3) by phosphorylating Rac1 at Ser71.
Product Information
Format
AlexaFluor®647
Presentation
Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 647 Conjugate is an antibody against Rac1 for use in Immunocytochemistry.
Key Applications
Immunocytochemistry
Application Notes
The unconjugated antibody (Cat. No. 05-389) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications.
Biological Information
Immunogen
Recombinant protein containing the full length human Rac.
Clone
23A8
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Recognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.
Isotype
IgG2b
Species Reactivity
Rat
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NM_006908
Gene Symbol
RAC1
TC25
MIG5
Purification Method
Affinity Purfied
UniProt Number
P63000
Molecular Weight
~21 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Rac1 in HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
