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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC | M | AlexaFluor®488 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-623-AF488 |
| Description | Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 488 Conjugate |
| Alternate Names |
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| Background Information | DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Presentation | Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in HeLa cells. The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II. |
| Epitope | carboxyl-terminal domain of RNA polymerase II |
| Clone | CTD4H8 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | This antibody recognizes Phospho- and non-phospho-RNA polymerase II. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Yeast, Rat based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~210-220 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in A431 cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in A431 cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |