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Millipore Anti-Nitrotyrosine Alexafluor® 647 Conjugate, Cat. No. 06-284-Af647, Is A Rabbit Polyclonal Antibody That Detects Tyrosine Nitrated Proteins And Has Been Tested For Use In Immunocytochemistry. - Mill (Additional S&H or Hazmat Fees May Apply)
Nitrotyrosine is produced by the action of reactive nitrogen species, such as peroxinitrite and nitrogen dioxide on tyrosine residues in proteins. Nitrotyrosine is an indicator of cell damage, inflammation, and nitric oxide production. Both free tyrosine and protein bound tyrosine residues are prone to nitration. In several pathological conditions, oxidative stress is increased resulting in the formation of super oxide and nitric oxide that react to form peroxynitrite (ONOO-), which is a highly reactive molecule. It causes oxidation of proteins and lipids and DNA damage, which results in loss of protein and lipid function and can increase the probability of gene mutations. Higher incidence of protein nitration has been reported in diabetes, atherosclerotic plaques, and rheumatoid arthritis, among others. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected cells and tissues by immunocytochemical techniques using antibodies developed against the nitrotyrosine.
Product Information
Format
AlexaFluor®647
Presentation
Purified rabbit polyclonal antibody in buffer containing PBS, pH7.1, and 15 mg/mL BSA with 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Nitrotyrosine AlexaFluor® 647 Conjugate, Cat. No. 06-284-AF647, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.
Key Applications
Immunocytochemistry
Application Notes
The unconjugated antibody (Cat. No. 06-284) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation.
Biological Information
Immunogen
Nitrated Keyhole Limpet hemocyanain (KLH)
Epitope
Nitrated Tyrosine
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects tyrosine nitrated proteins.
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with all species.
Antibody Type
Polyclonal Antibody
Purification Method
Affinity Purfied
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in A431 treated with peroxytnitrite.
Immunocytochemistry Analysis: 1:100 dilution of this antibody detected Nitrotyrosine in A431 cells treated with 5 mM peroxytnitrite.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
